QIAGEN

NTA Agarose

For efficient immobilized-metal affinity chromatography (IMAC) using gravity-flow chromatography

·         High binding affinity and high capacity

·         Choice of purification under native or denaturing conditions

·         Fine-tuning of purification strategies through flexible choice of metal ion

Uncharged NTA Agarose allows researchers to choose the metal ion they use for IMAC procedures,

allowing fine-tuning of purification strategies. Metal ions such as Ni ²⁺, Cu ²⁺, Zn²⁺, or Co²⁺ are

efficiently immobilized for IMAC purification of metal-binding proteins. NTA has four chelating sites to

bind metal ions more tightly than other metal-chelating purification systems.

This reduces metal-ion leaching and nonspecific binding and leads to increased protein binding capacity and purer preparations.

A proprietary spacer that links NTA to the sepharose support provides optimal accessibility to metal ions, increasing protein-binding capacity.

Feature	Specifications
Applications	Proteomics
Bead size	45-165 µm
Binding capacity	Up to 50 mg/ml (up to 2.5 µmol @ ~20 kDa* * Based on immobilized nickel ion chromatography; capacities may vary for other ions.
Form	50% suspension in 30% ethanol
FPLC	Yes
Gravity flow or spin column	Gravity flow
Processing	Manual/Automated
Scale	Large scale
Special feature	Batch and column purification
Start material	Cell lysate
Support/matrix	Sepharose CL-6B
Yield	100 µg - 100 mg

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