QIAGEN

N-Terminus pQE Vector Set

For high-level expression of N-terminally His-tagged proteins

·         pQE-30 vectors with MCS in all three reading frames for fast cloning

·         pQE-40 vector for poorly expressed proteins, short peptides

·         pQE-40 vector expresses DHFR-fusions, enhancing stability and antigenicity

This set provides 5 vectors (pQE-9, pQE-30, pQE-31, pQE-32, and pQE-40) for expression of N-terminally His-tagged proteins.

pQE-30, pQE-31, and pQE-32 provide the multiple cloning site (MCS) in all three reading frames while pQE-9 has an alternative, shorter multiple cloning site.

pQE-40 is designed for expression of DHFR-fusion proteins and is recommended for expression of poorly expressed proteins or short peptides, which are often prone to proteolysis, as DHFR enhances both stability and antigenicity.

Since DHFR itself displays little immunogenicity in mouse and rat, DHFR-fusion proteins are ideal for epitope screening.

Feature	Specifications
All three reading frames provided	Yes
Expression	In vivo
Expression species	E. coli
In-frame cloning necessary	Yes
N- or C-terminal tag	N-terminal tag
Tag	6xHis tag
Tag removal sequence	No

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