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MinElute Reaction Cleanup Kit

 

 

For cleanup of up to 5 ?g DNA (70 bp to 4 kb) from enzymatic reactions

 

·         Very small elution volumes

·         Fast procedure and easy handling

·         High, reproducible recoveries

·         Gel loading dye for convenient sample analysis

The MinElute Reaction Cleanup Kit provides spin columns, buffers, and collection tubes for silica membrane-based purification of DNA 70 bp - 4 kb in size from enzymatic reactions.

The spin columns are designed to allow elution in very small volumes (as little as 10 ?l), delivering highly concentrated DNA in high yields.

An integrated pH indicator allows easy determination of the optimal pH for DNA binding to the spin column. The procedure can be fully automated on the QIAcube.

DNA fragments purified with the MinElute system are ready for direct use in all applications, including sequencing, microarray analysis, ligation and transformation, restriction digestion, labeling, microinjection, PCR, and in vitro transcription.

Feature

Specifications

Binding capacity

5 µg

Elution volume

10 µl

Format

Tube

Fragment size

70 bp - 4 kb

Processing

Manual

Recovery: oligonucleotides dsDNA

Recovery: oligonucleotides, dsDNA

Removal <10mers 17-40mers dye terminator proteins

Removal <40mers

Sample type: applications

DNA, oligonucleotides: Enzymatic reactions

Technology

Silica technology

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

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TEL/FAX: 01 (222) 2198815, 274 5072, 274 5073

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