For reliable purification of GST-tagged proteins
- New glutathione matrix with 50% increased binding capacity
- Reliable dynamic binding up to 20 mg/ml
- High flow characteristics
- Mild elution conditions maintain protein function
- Excellent choice for column purification and scale-up
Glutathione HiCap represents a state-of-the-art matrix for affinity purification of GST and GST-fusion protein.
Purification of these GST-tagged proteins is achieved by their strong and specific binding to the tripeptide ligand glutathione, which is stably immobilized on the bead support of the matrix.
Glutathione HiCap Matrix provides high binding capacity for GST-tagged proteins and ensures high-resolution affinity purification of GST and GST-fusion proteins.
The
Glutathione HiCap Matrix is available in both bulk and cartridge formats.
|
Feature |
Specifications |
|
Applications |
Proteomics |
|
Binding capacity |
up to 12 mg /ml |
|
Gravity Flow or FPLC |
Glutathione Superflow: gravity flow or FPLC;
Gluthatione Superflow Cartridge: FPLC or
syringe |
|
N- or C-terminal tag |
N-terminal |
|
Processing |
manual/automated |
|
Scale |
Glutathione Superflow: large scale; Gluthatione
Superflow Cartridge: medium scale |
|
Special feature |
tag increases protein
solubility |
|
Start material |
cell lysate |
|
Support/matrix |
Superflow |
|
Tag |
GST-tag |
|
Yield |
uo to 12
mg/ml |
