QIAGEN

FastLane Cell SYBR^® Green Kit

For SYBR Green real-time one-step RT-PCR analysis from cultured cells without RNA purification

·         No RNA purification required, significantly saving time

·         Just 3 steps from cells to real-time RT-PCR

·         High-throughput analysis of 96- and 384-well plates

·         Unique gDNA Wipeout Buffer enables detection of RNA only

·         Immediate startup using optimized reagents and protocols

The FastLane Cell SYBR Green Kit accelerates and streamlines real-time RT-PCR and is ideal for experiments requiring rapid high-throughput gene expression analysis.

The kit provides a set of buffers for preparing FastLane lysates from cultured cells.

In addition to lysing cells, the buffers also stabilize cellular RNA and eliminate genomic DNA. FastLane lysates are used directly in SYBR Green-based real-time one-step RT-PCR.

A variety of other detection formats are also available, including singleplex detection with sequence-specific probes or multiplex detection with sequence-specific probes (with or without ROX passive reference dye).

Feature	Specifications
Applications	SYBR Green-based, real-time, one-step RT-PCR
Enzyme activity	Primer annealing
Mastermix	Yes
Reaction type	One-step, cell lysis, RNA stabilization, DNA digestion
Real-time or endpoint	Real time
Sample/target type	Cultured cells
Single or multiplex	Single
With/without hotstart	Without hotstart

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QIAGEN

FastLane Cell SYBR® Green Kit

For SYBR Green real-time one-step RT-PCR analysis from cultured cells without RNA purification

FastLane Cell SYBR^® Green Kit