QIAGEN

FastLane Cell Probe Kit

For probe-based real-time one-step RT-PCR analysis from cultured cells without RNA purification

·         No RNA purification required, significantly saving time

·         Just 3 steps from cells to real-time RT-PCR

·         High-throughput analysis of 96- and 384-well plates

·         Unique gDNA Wipeout Buffer enables detection of RNA only

·         Immediate startup using optimized reagents and protocols

The FastLane Cell Probe Kit accelerates and streamlines real-time RT-PCR and is ideal for experiments requiring rapid high-throughput gene expression analysis.

The kit provides a set of buffers for preparing FastLane lysates from cultured cells. In addition to lysing cells, the buffers also stabilize cellular RNA and eliminate genomic DNA.

FastLane lysates are used directly in probe-based real-time one-step RT-PCR.

A variety of other detection formats are also available, including detection with SYBR Green or multiplex detection with sequence-specific probes (with or without ROX passive reference dye).

Feature	Specifications
Applications	Probe-based, real-time, one-step RT-PCR
Enzyme activity	Primer annealing
Mastermix	Yes
Reaction type	One-step, cell lysis, RNA stabilization, DNA digestion
Real-time or endpoint	Real time
Sample/target type	Cultured cells
Single or multiplex	Single
With/without hotstart	Without hotstart

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