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FastLane Cell Multiplex Kits

 

 

 

For multiplex, real-time one-step RT-PCR analysis from cultured cells without RNA purification

 

·         No RNA purification required, significantly saving time

·         Just 3 steps from cells to real-time RT-PCR

·         High-throughput analysis of 96- and 384-well plates

·         Unique gDNA Wipeout Buffer enables detection of RNA only

·         Immediate startup using optimized reagents and protocols

 

FastLane Cell Multiplex Kits accelerate and streamline real-time RT-PCR and are ideal for experiments requiring rapid high-throughput gene expression analysis.

The kits provide a set of buffers for preparing FastLane lysates from cultured cells. In addition to lysing cells, the buffers also stabilize cellular RNA and eliminate genomic DNA.

FastLane lysates are used directly in multiplex, real-time one-step RT-PCR (with or without ROX passive reference dye).

A variety of other detection formats are also available, including detection with SYBR Green or sequence-specific probes.

Feature

Specifications

Applications

Multiplex, real-time, one-step RT-PCR

Enzyme activity

Primer annealing

Mastermix

Yes

Reaction type

One-step, cell lysis, RNA stabilization, DNA digestion

Real-time or endpoint

Real time

Sample/target type

Cultured cells

Single or multiplex

Multiplex

With/without hotstart

Without hotstart

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

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TEL/FAX: 01 (222) 2198815, 274 5072, 274 5073

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