QIAGEN

Factor Xa Protease

For endoproteolytic removal of short 6xHis affinity tags from 6xHis-tagged recombinant proteins

· Efficient 6xHis tag cleavage: >90%

· Protein free of vector-encoded amino acids from pQE-30 Xa vector

Factor Xa Protease is a site-specific endoprotease that preferentially cleaves the C-terminal peptide bond of the recognition sequence Ile-Glu-Gly-Arg.

Therefore, Factor Xa Protease efficiently cleaves 6xHis-tagged proteins expressed by the pQE-30 Xa vector as the 6xHis tag precedes the recognition sequence, giving protein free of vector-encoded amino acids.

After cleavage of the purified protein, Factor Xa Protease is removed in a batch procedure using Xa Removal Resin.

Feature	Specifications
Applications	Protein crystallography, structure determination studies using NMR
Efficiency of Tag removal	>90%
Protease recognition site	Factor Xa Protease recognition site
Special feature	Protein free of vector-encoded amino acids
Tag removal	Endoproteolytic

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