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QIAGEN

 

DyeEx 96 Kit

 

 

For removal of unincorporated dye terminators from 1-96 sequencing reactions

 

·         Fast procedure with only two short centrifugation steps

·         Ready-to-use prehydrated gel-filtration material

·         Efficient removal of any dye terminator

DyeEx Kits provide either spin columns or 96-well plates and use gel-filtration technology to remove dye terminators from sequencing reactions.

 

Sequencing reactions are loaded onto the pre-hydrated gel-filtration material.

 

After a short centrifugation step, the reactions are ready to be loaded onto a capillary sequencer. Unincorporated dye terminators are retained in the gel matrix

 

Feature

Specifications

Binding capacity

10–20 µl

Elution volume

10–20 µl

For DyeEx Kits:

Terminator removal, ABI PRISM 377, 373, 310, 3100, or 3700, MegaBACE 1000, or CEQ 2000 sequencers

Format

96-well plate

Processing

Manual

Removal <10mers 17–40mers dye terminator proteins

Dye terminator

Sample type: applications

Sequencing reactions

Technology

Gel filtration

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

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