QIAGEN

C-Terminus pQE Vector Set

For high-level expression of C-terminally 6xHis-tagged proteins

·         C-terminal 6xHis tag ensures that only full-length protein is purified

·         pQE-16 vector for expression of poorly expressed proteins as DHFR fusions

·         pQE-16 vector for expression of short peptides as DHFR fusions

This set provides 3 vectors (pQE-16, pQE-60, and pQE-70) for expression of C-terminally 6xHis-tagged proteins and is recommended for open reading frames with “pause sites”, which can cause premature termination.

pQE-60 and pQE-70 allow the original start codon of the coding fragment to replace the ATG in the pQE vector, preserving the authentic N-terminus of the protein.

These two constructs are created by introducing anNcoI and a SphI restriction site, respectively, at the ATG codon of the insert by PCR or mutagenesis.

pQE-16 allows expression of C-terminally 6xHis-tagged DHFR-fusion proteins.

DHFR (dihydrofolate reductase) enhances antigenicity and stability, and is recommended for poorly expressed proteins or short peptides prone to proteolysis. Since DHFR itself displays little immunogenicity in mouse and rat, DHFR-fusion proteins are ideal for epitope screening.

Feature	Specifications
All three reading frames provided	Yes
Expression	In vivo
Expression species	E. coli
In-frame cloning necessary	Yes
N- or C-terminal tag	C-terminal tag
Special features	Based on the T5 promoter transcription-translation system
Tag	6xHis tag
Tag removal sequence	No

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